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How do you make phosphate buffered saline?

  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 8 g of Sodium chloride to the solution.
  3. Add 0.2 g of Potassium Chloride to the solution.
  4. Add 1.44 g of Sodium Phosphate Dibasic to the solution.
  5. Add 0.245 g of Potassium Phosphate Monobasic to the solution.

How do you dilute 10X PBS to prepare 1X PBS 500 mL final volume?

Diluting a 10X Solution to Make 1X PBS A 5X solution must be diluted 5 times to make a normal dilution, while a 10X solution must be diluted 10 times. To prepare a 1 liter working solution of 1X PBS from a 10X PBS solution, add 100 ml of the 10X solution to 900 ml of water.

How do you make 0.1 M phosphate buffer saline solution?

ADD 900 ml of water ,stir until the powder dissolved . Using the PH meter adjust the PH to 7.4 using NAOH or HCL . TRANSFER the liquid to a 1 liter VOL. flask , add enough water to make 1 lit.

How do you dilute 20X PBS to 1X PBS?

To make a 1X PBS solution dilute concentrate 20X with distilled water. Measure and pour appropriate volume of 20X PBS concentrate into a mixing flask and add DI water to final volume. Stir briefly. The 1X solution should be pH 7.6 ± 0.2.

How do you make a phosphate buffer solution?

  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 20.214 g of Sodium Phosphate Dibasic Heptahydrate to the solution.
  3. Add 3.394 g of Sodium Phosphate Monobasic Monohydrate to the solution.
  4. Adjust solution to final desired pH using HCl or NaOH.
  5. Add distilled water until the volume is 1 L.

How do I make PBS 10X?

To make 1 L of 10X PBS stock solution, combine 17.8 g of Na2HPO4, 2.4 g of KH2PO4, 80 g of NaCl, 2 g of KCl, and adjust final volume to 1 L. This recipe calculator enables the accurate preparation of 10X PBS for any millimeter volume.

What is the difference between 1X and 10X PBS?

PBS should be 1x in order to maintain the osmolarity of the cells. 10x pbs can result in the cell shrinking or swelling of the cells which will eventually distort the cell morphology that will lead to the discrepancy in IF protocol.

How do you make a 1 M phosphate buffer?

Potassium Phosphate Buffer (1 M, pH 6.5) Solution Preparation and Recipe

  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 95 g of Potassium Phosphate Monobasic to the solution.
  3. Add 52.5 g of Potassium phosphate dibasic to the solution.
  4. Adjust the pH to 6.5.
  5. Add distilled water until the volume is 1.

How do you make a 0.2 M sodium phosphate buffer?

Dissolve 35.61 g of Na2HPO4•2H2O and 27.6 g of NaH2PO4•H2O separately in H2O. 2. Adjust the volume of each solution to 1000 mL. Store the stock solutions for up to 6 mo at 4°C.

How do I make a 20X PBS buffer?

20X PBS Stock Solution

  1. 2.88 g Na2HPO.
  2. 160.00 g NaCl.
  3. 4.00 g KH2PO.
  4. 4.00 g KCl.

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